Gene Project

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Title

Sequence

Protein DNA/RNA

Sequence Length(序列长度): 5'- Franking Sequence: 3'- Franking Sequence:

Target Expression Host:

ChooseSpecie

高表达密码子表(推荐使用) type_2	CUTG_2007 type_1	自定义表 type_0
Codon Table for High Expression(recommended)		Customized Codon Table

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Individual Codon Usage

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Codon Context

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Codon Adaptation Index

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Number of Hidden Stop Codons

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mRNA Structure

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GC Content:

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Optional: Remove Repeats:

Length of Nucleotide Motif:

Minimum Number of Repeats:

Restriction enzyme sites

Other customized sequence motif

Cis-acting elements

hostName	enzymeName&enzymeSequence
原核生物（大肠肝菌，芽孢杆菌等）	RBS:AGGAGG;Chi_sites:GCTGGTGG;T7Cis:ATCTGTT;SD_like:GGRGGT;PolyA:AAAAAAA;PolyT:TTTTTT;
昆虫类（昆虫，果蝇，线虫，家蚕等）	Splice:GGTAAG;Splice:GGTGAT;PolyA:AATAAA;PolyA:AAAAAAA;PolyT:TTTTTT;
植物类（拟南芥，玉米，烟草，蕃茄等）	Splice:GGTAAG;Splice:GGTGAT;Splice:GTAAAA;Splice:GTAAGT;Splice:GTACGT;PolyA:AATAAA;PolyA:AATGAA; PolyA:AATGGA;PolyA:TATAAA;PolyA:AATAAT;Destabilizing:ATTTA;PolyT:TTTTTT;PolyA:AAAAAAA;
其他真核生物（酵母，人，哺乳动物， CHO细胞等）	Splice:GGTAAG;Splice:GGTGAT;PolyA:AATAAA;PolyA:ATTAAA;PolyA:AAAAAAA;PolyT:TTTTTT; Destabilizing:ATTTA;